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 The EPC 10 is fully integrated with the new LIH 1600 AD/DA interface. The advanced integration of the EPC 10 with the A/D D/A interface and software minimizes total recording noise, eliminates compatibility problems, saves on additional equipment expenses and set-up and training times. The built-in interface is connected to the PCI-1600 computer interface card via fiber optic data cables. This connection provides superb optical isolation, virtually eliminating ground loops and increasing the distance between the computer and the amplifier by up to 5 meters. The LIH 1600 has eight analog inputs, four analog outputs, sixteen digital inputs and sixteen digital output channels all sampling synchronously. Additionally, there are two "telegraphing" channels for monitoring external devices. In current clamp (CClamp) mode, the membrane potential (Vm) is measured and used to compute a current waveform which is fed back into the cell. As a result, slow voltage drift and other artificial voltage-dependent conductances are controlled and adjusted to a fixed value. This Low Frequency Voltage Clamp (LFVC) negates any readjustment of the holding current while conducting CClamp experiments. A new design of the pre-amplifier allows to perform "True CClamp" experiments. In CClamp mode, the headstage acts as a voltage follower circuit, which guarantees very fast and accurate membrane potential recordings. Similar to a classical microelectrode amplifier, the cell voltage is measured on a very high input resistance and C-fast compensation is no longer necessary. |
Using novel components and a new arrangement of the parts on a multi layer board, we drastically reduced
the dimensions of the headstage. The slim shape of the completely new designed headstage allows optimal access
to your cells. The connection to the pipette holder is achieved via a stable BNC connector. Adapter plates for various micromanipulators are available from HEKA. 
The EPC 10 is equipped with 3 TTL trigger outputs and 1 trigger input on the front panel. There are 16
additional digital outputs and 16 digital inputs available at the rear panel of the EPC 10. The digital
outputs can be used to control external devices like perfusion systems or video imaging systems. Two
telegraphing inputs on the rear panel can be used to combine the EPC 10 amplifier with external devices,
e.g. telegraphing gain amplifiers.
Like the EPC 9, the versatility of the EPC 10 stems from it’s adjustment and
control at the software level. Leak currents, capacitances, and series resistance can instantly be neutralized
automatically while you still retain the possibility of full manual control of the amplifier. Voltage- and
Current-Clamp experimental protocols are easily automated; not only ensuring simplicity, great speed and
accuracy but also providing enormous flexibility for the integration of extensions such as photometric
and fluorescence measurements.
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