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 The EPC 10 USB is fully integrated with HEKA’s LIH 8+8 AD/DA interface. The advanced integration of the EPC 10 USB with the A/D D/A interface and software minimizes total recording noise, eliminates compatibility problems, saves on additional equipment expenses and set-up and training times. The built-in interface utilizes the latest USB 2.0 and high speed processing technologies without the need for a peripheral PCI card. The analog input and output channels are isolated from the digital lines that communicate with the computer. Each analog channel has its own separate ground patch and the digital section has a completely different ground. The result is complete isolation of the acquisition side from the computer side eliminating ground loops and achieving low noise. The LIH 8+8 has eight analog inputs, four analog outputs, sixteen digital inputs and sixteen digital output channels all sampling synchronously. In current clamp (CClamp) mode, the membrane potential (Vm) is measured and used to compute a current waveform which is fed back into the cell. As a result, slow voltage drift and other artificial voltage-dependent conductances are controlled and adjusted to a fixed value. This Low Frequency Voltage Clamp (LFVC) negates any readjustment of this holding current while conducting CClamp experiments. The design of the pre-amplifier allows “True CClamp” experiments to be performed. In CClamp mode, the headstage acts as a voltage follower circuit, which guarantees very fast and accurate membrane potential recordings. Similar to a classical microelectrode amplifier, the cell voltage is measured on a very high input resistance and C-fast compensation is no longer necessary |
The EPC 10 USB is equipped with 3 TTL trigger outputs and 1 trigger input on the front panel. There are 16
additional digital outputs and 16 digital inputs available at the rear panel of the EPC 10 USB. The digital outputs
can be used to control external devices like perfusion systems or video imaging systems.
The versatility of the EPC 10 USB originates from its adjustment and control capabilities at the software level.
Leak currents, capacitances, and series resistance can instantly be neutralized automatically while you still retain
the possibility of full manual control of the amplifier. Voltage- and Current-Clamp experimental protocols are
easily automated; not only ensuring simplicity, great speed and accuracy but also providing enormous flexibility
for the integration of extensions such as photometric and fluorescence measurements.
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