PK2+ pipette perfusion kit

The 2PK+ is a kit designed to perfuse patch pipettes during single channel recording and to internally perfuse cells during whole-cell recordings. The 2PK+ allows for the change of solution inside the pipette as well as inside the whole-cell itself. This system can be combined with the MRC-6, Multi-Reservoir Carousel, for multiple changes of internal pipette solutions when perfusing with the 2PK+. The kit includes a precise pressure/vacuum generator, perfusion pipette holder (specify amplifier used), perfusion pressure vessel, and quartz perfusion capillaries. The generator will control and measure the pressure/vacuum with an accuracy of +/- 0.2mmHg throughout the range of +/-50mmHg.

A precise pressure / vacuum generator forces the perfusion solution via quartz microcapillaries into the patch pipette. The ballance between positive and negative pressure allows the cell to experience no net change in pressure.

Features:
  • Precise pressure / vacuum generator, accuracy ±0.2 mm Hg over a range of ±40 mm Hg, requires a gas input of 15 PSI / 100 kPa
  • Low-noise remote power supply
  • Pipette holders available for all common patch-clamp amplifiers
  • Pressure vessel
  • Quartz perfusion capillaries
  • Butane torch

optional:

  • MRC-6 multi-reservoir carousel for up to 6 different solutions

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