HEKA Tutorials

Hardware
Installation of Hardware and Software (Installation Guide)	Instructions, how to install the HEKA hardware (EPC 10, LIH 1600) and software (PULSE, PATCHMASTER, driver software).
Installation of Hardware and Software (Installation Guide v8.5)	Instructions, how to install the HEKA hardware (EPC 9, ITC-16, PCI-16) and software (PULSE, E9Screen, driver software).
Testing the EPC 10 with the Model Circuit (EPC 10 Manual: "Verifying and Testing the EPC 10")	A first test of the amplifier and a introduction to the compensation routines of the EPC 10 patch clamp amplifier.
Testing the EPC 9 with the Model Circuit (EPC 9 Manual: "Testing the EPC9 with the Model Circuit")	A first test of the amplifier and a introduction to the compensation routines of the EPC 9 patch clamp amplifier.
PATCHMASTER		Download
The following documents are part of the PATCHMASTER Tutorial		Download a demo configuration for this tutorial
The First Experiment (PATCHMASTER Tutorial)	This tutorial will guide you through the main features of the PATCHMASTER program. It briefly describes how a very simple first experiment with PATCHMASTER could look like.
Chart Recording with PATCHMASTER's Online Analysis (PATCMASTER Tutorial)	The Online Analysis of PATCHMASTER together with the Protocol Editor provide features for a chart display.		PATCHMASTER 2.20 (updated: 12-Dec-06)
Cm-Measurements with PATCHMASTER (PATCMASTER Tutorial)	The software LockIn amplifier of PATCHMASTER together with an EPC 10 or EPC 9 comprises a unique "virtual" instrument with unprecedented capabilities for performing membrane capacitance"
Fluorescence Measurements with PATCHMASTER (PATCMASTER Tutorial)	In this tutorial we will describe how to perform a ratiometric fluorescence measurement during a whole-cell experiment and how to perform high-speed fluorescence measurements simultaneously to a current recording.
The following documents are part of the PATCHMASTER Reference Manual (Part II)		Download a demo configuration for this tutorial
PATCHMASTER for PULSE users (PATCMASTER focus document)	This document is intended for those users familiar with the PULSE acquisition software. We will quickly summarize the most relevant differences between PULSE and PATCHMASTER.
Export of PATCHMASTER data to PULSE data format (PATCMASTER focus document)	The PATCHMASTER data format has been significantly extended compared to the PULSE data format. Therefore, conversion of PATCHMASTER data into PULSE data format only works for data that have been acquired following the more restrictive conventions of PULSE.
The "global variables" of PATCHMASTER (PATCMASTER focus document)	We will shortly outline how these global variables can be used, which features they enable, and how they can facilitate various task.
Non-stored ("conditioning") segments in PATCHMASTER (PATCMASTER focus document)	The purpose of this feature is to save space when long conditioning intervals have to be introduced between important data and when the data of such conditioning segments are not of interest.
Using a Recorded Waveform as Stimulus with PATCHMASTER (PATCMASTER focus document)	The stimulus output by PATCHMASTER can be either computed by the program or loaded from a file instead if you activate "load from file template"
Additional Demo Configurations for PATCHMASTER
Continuous Recording	Continuous data recording (for minutes or even hours) in voltage clamp mode and in current clamp mode. Changing the holding potential in continuous recordings. Setting markers in continuous recordings.		PATCHMASTER 2.20 (updated: 25-Jan-07) PATCHMASTER 2.20 (updated: 25-Jan-07)
Ramp Protocols and I/V Plots	Stimulating with Ramp protocols. Current versus Voltage plots from Ramp data. Displaying traces in the Online Analysis.		PATCHMASTER 2.20 (updated: 25-Jan-07) PATCHMASTER 2.20 (updated: 25-Jan-07)
Linking PGF sequences	Linking of PGF sequences in the protocol editor. Switching from VC to CC mode and vice versa in a protocol. Using the “Build Instruction” in a PGF sequences. Display of data traces in a online window.		PATCHMASTER 2.20 (updated: 26-Jan-07) PATCHMASTER 2.20 (updated: 26-Jan-07)
Membrane capacitance and stimulation	Stimulate with artificial action potential and measure membrane capacitance before and after the action potential Geberate trains of action potentials Use pgf parameters to parameterize the action potential (train) Record an action potential (train) in current clamp and save it as "stimulus template" Combine membrane capacitance measurement with stimulus templates from a file		PATCHMASTER 2.20 (updated: 12-Dec-06) PATCHMASTER 2.20 (updated: 12-Dec-06)
Online Averaging	Use the Trace Buffers for online averaging of traces Show the average trace in the online analysis window Save the average trace using the "Replace Target" function Average the recorded sweeps using the "Average Sweeps" function Subtraction of a reference trace (for diaplay and analysis)		PATCHMASTER 2.20 (updated: 12-Dec-06) PATCHMASTER 2.20 (updated: 12-Dec-06)
FURA and LockIn	Combined fura-2 (calcium) and membrane capacitance recordings Use of the Photometry Wave Chart recording: "share x-axis" function in the analysis dialog Trace mapping concept: A trace keeps its index independent from order of acuisition or number of traces recorded		PATCHMASTER 2.20 (updated: 13-Oct-07)
Automatic Stimulus Adjustment in Current Clamp Mode	Triggering of action potentials (APs) by injecting a current pulse that is just large enough to elicit an AP. Setting acquisition parameters using pgf-parameters. Responding on feedback from online analysis. Absolute and Relative stimulation to I-membrane.		PATCHMASTER 2.20 (updated: 12-Dec-06) PATCHMASTER 2.20 (updated: 12-Dec-06)
On-cell Cm measurements using the EPC 10 Neef A, Heinemann C, Moser T. "Measurements of membrane patch capacitance using a software-based lock-in system." Pflugers Arch. 2007 Jan 6;	On-Cell membrane capacitance measurements. Using the software lock-in at high sine wave frequencies. Manual lock-in calibration (phase and attenuation determination. Links: A Software Lock-in Amplifier for On-Cell Membrane Capacitance Measurements Kavli Workshop in Pucon: "Advanced Techniques to Study Single Vesicle Exocytosis." Organizers: Manfred Lindau, Cornell University; Ricardo Borges, La Laguna University. eBriefing of The New York Academy of Science, posted 10. July 2006. Summary Slides High Resolution On-Cell Capacitance Measurements with a Software Lock-In System IGOR files for offline analysis of Oncell Cm data acquired with Patchmaster		PATCHMASTER 2.20 (updated: 30-Jan-07) PATCHMASTER 2.20 (updated: 30-Jan-07)
Fast Solution Change within a Sweep Recording	Studying ligand-gated ion channels. Opening and closing a valve during a sweep recording. Controlling a fast perfusion system with a digital word output. Changing the order of valve opening in a recording. Use “values” and “pgf-parameters” to control the digital outputs. Performing multiple solution applications in one sweep. Acquiring multiple sweeps with different solution applications. Use of the “Collapse” functions to convert multiple series with one sweep into one series with multiple sweeps.		PATCHMASTER 2.20 (updated: 25-Jan-07) PATCHMASTER 2.20 (updated: 25-Jan-07)
Rapid Mode Switching	Switching from VC mode to CC mode or vice versa during acquisition.		PATCHMASTER 2.20 (updated: 04-Apr-07)
Controlling Patchmaster from another application	Controlling Patchmaster from another program via the “batch control” interface.		PATCHMASTER 2.20 (updated: 04-Apr-07)
Impedance Spectroscopy with Patch Clamp Amplifiers	Using the lock-in at different frequencies. Using the ScriptCommander for acquisition of frequency scans. Automatic Lock-in calibration for multiple frequencies. Please note that the ScriptCommander program is required. It is included in the sample configuration "Controlling Patchmaster from another application"		PATCHMASTER 2.20 (updated: 04-Apr-07)
PULSE+PULSEFIT
Starting PULSE (PULSE manual: "The first experiment")	The first steps at the beginning of a typical patch clamp experiment.
The "Get File Template" Feature (PULSE Manual: "The "Get File Template" Feature")	The "Get File Template" feature can be used to apply a complex, user defined wave template (e.g. a pre-recorded action potential) as stimulus signal.
Capacitance measurements in oocytes Bernhard M. Schmitt, Universität Würzburg	Real-time monitoring of membran capacitance in Xenopus oocytes with PULSE and X-Chart.		PULSE + X-Chart (updated: 22-Oct-02) PULSE + X-Chart (updated: 22-Oct-02)
Membrane capacitance (Cm) measurements with EPC 9 and PULSE (LockIn Manual)	A step-by-step tutorial, describing the usage of the software LockIn amplifier in membrane capacitance measurements.
Please note: All HEKA manuals can be downloaded from the Download section of our web site.